Proteins - Determining enzyme selectivity
3 important questions on Proteins - Determining enzyme selectivity
How did they go form peptide analysis to selectivity?
They looked at the amount of cleaved products over time. This could be determent with the new way of peptide analysis (LC with UV).
This showed that a certain enzyme might cleave a certain bond much faster then others. A rate can be determent form this.
This showed that a certain enzyme might cleave a certain bond much faster then others. A rate can be determent form this.
What did they found out about selectivity and how can you couple this with digestibility of a enzyme?
They found out that if you had charged AA around the cleavage side the cutting would be more slow. So if you now have the sequence of a protein you can predict if the enzyme is cutting it fast or slow.
What is the difference between enzyme specificity and enzyme selectivity?
Enzyme specificity is the AA aftehr which the enzyme can cleave the peptide bond.
Enzyme selectivity is the rate of hydrolysis of each cleavage site. Compared to the total sum of rates of hydrolysis of all cleavage sites.
So specificity is about where the cutting of the peptide bond can happen and slectivity is about how fast it can go
Enzyme selectivity is the rate of hydrolysis of each cleavage site. Compared to the total sum of rates of hydrolysis of all cleavage sites.
So specificity is about where the cutting of the peptide bond can happen and slectivity is about how fast it can go
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