Analysis of Carbohydrates - Examples of carbohydrate analysis
3 important questions on Analysis of Carbohydrates - Examples of carbohydrate analysis
How do you do a dietary fiber analysis?
First you extract the fibers and then you separate them in 2 fractions. The alcohol soluble solids (ASS) and the alcohol insoluble solids (AIS). You hydrolyse them and analyse them with HPLC or GLC.
How does the AOAC method for GOS analysis work?
- GOS is extracted in hot water
- GOS is hydrolyzed with beta-galactosidase
- Sample is analysed with HPAEC
- Also blank sample without enzyme treatment is analysed with HPAEC (in order to compensate for the natural present lactose)
What are the advantages and limitations of the AOAC GOS method?
Advantages:
Limitations
- The only validated & official method
- Easy protocol
- Possible for most laboratories
Limitations
- No information on the individual compounds of GOS
- Assumed knowledge on GOS DP
- Potential error in products containing high levels of free galactose or lactose
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