Food traceability and authentication - ELISA essay on proteins
5 important questions on Food traceability and authentication - ELISA essay on proteins
How does ELISA work?
The test is very simple and suited to large scale screening, but not very robust in identification and quantification, easily gives false positives or false negatives
Very much dependent on the quality of antibodies
Processing easily alter the results
Sensitivity in the low ppm range
How does the sandwich ELISA work?
How does a indirect ELISA work?
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What are the weaknesses of a ELISA?
- Small linear range
- Easily affected by processing and by the matrix
- High risk of false positives or false negatives
- Very careful calibration , matrix-matched and process-mached, is always needed
What are the strengths of a ELISA?
- Fast, easy and cheep
- Reliable in carefull controlled and calibrated recetitive analyses
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