Summary: Advanced Cellular Imaging Techiques
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2 Microscope Construction
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2.1 Compound microscope
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A compound microscope reaches higher magnification by...
Using extra lenses, such as an eyepiece. -
2.2 Infinity optical system
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Infinity optical system
Here there are two lenses, but no intermediate-image.
- Object is placed in the focal plane (s=f, s'=infinity)
- Each point of the object forms a parallel bundle
- A tubelens forms a real image on a camera sensor. -
2.4 Objective Lenses
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Infinity sign on objective lens
Optimized for an infinity optical system -
Magnification on objectiive lens
Total magnification -
2.5 Numerical aperture
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Numerical aperture of an objective is...
A measure of its ability to gather light and to resolve detail in a specimen. -
High N.A. Lenses... (2)
1. Collect more light and formbrighter images
2. Allow to see more detail -
Formula N.A.
NA = sin mu -
Maximal and best lenses N.A.
Maximal = 1, Best = 0.95 -
Mu increases when... (2)
1. The aperture of the lens is larger
2. Distance to the sample is minimized -
Image brightness formula
NA^2
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Topics related to Summary: Advanced Cellular Imaging Techiques
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Resolution and contrast generation - Sample illumination by a condenser lens
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Resolution and contrast generation - Field diaphragm
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Resolution and contrast generation - Dark field microscopy
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Resolution and contrast generation - Phase contrast microscopy
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Resolution and contrast generation - Polarized light microscopy
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Resolution and contrast generation - Differential Interference Contrast (DIC)
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Fluorescence microscopty - Fluorescence in General
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Fluorescence microscopty - Fluorescence contribution factors
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Fluorescence microscopty - Optical Sectioning Techniques
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Light-activated experiments and super-resolution imaging - Light-activated experiments
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Light-activated experiments and super-resolution imaging - Super Resolution; beating the diffraction limit - PALM
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Reporter genes - Different ways to monitor expression
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Reporter genes - Reporter proteins - Bioluminiscence
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Reporter genes - Reporter proteins - Fluorescence reporters
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Reporter genes - Getting the reporter gene in cells - Stable transformation
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Reporter genes - Getting the reporter gene in cells - Transient expression
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Fluorescence in Biology - Fluorescence in general
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Fluorescence in Biology - Fluorescent label
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Fluorescence in Biology - Ratiometric imaging
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Förster Resonance Energy Transfer (FRET)
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Techniques to determine FRET - Donor Fluorescence Lifetime (FLIM)
