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A snapshot of the summary - Advanced Cellular Imaging Techiques
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2 Microscope Construction
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2.1 Compound microscope
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A compound microscope reaches higher magnification by...Using extra lenses, such as an eyepiece.
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2.2 Infinity optical system
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Infinity optical systemHere there are two lenses, but no intermediate-image.
- Object is placed in the focal plane (s=f, s'=infinity)
- Each point of the object forms a parallel bundle
- A tubelens forms a real image on a camera sensor. -
2.4 Objective Lenses
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Infinity sign on objective lensOptimized for an infinity optical system
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Magnification on objectiive lensTotal magnification
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2.5 Numerical aperture
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Numerical aperture of an objective is...A measure of its ability to gather light and to resolve detail in a specimen.
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High N.A.
Lenses ... (2)1. Collect more light and formbrighter images
2. Allow to see more detail -
Formula N.A.NA = sin mu
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Maximal and best lenses N.A.Maximal = 1, Best = 0.95
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Mu increases when... (2)1. The aperture of the lens is larger
2. Distance to the sample is minimized -
Image brightness formulaNA^2
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