Summary Advanced Cellular Imaging Techiques

A snapshot of the summary - Advanced Cellular Imaging Techiques

• 2 Microscope Construction

• 2.1 Compound microscope

• A compound microscope reaches higher magnification by...

  Using extra lenses, such as an eyepiece.

• 2.2 Infinity optical system

• Infinity optical system

  Here there are two lenses, but no intermediate-image.
  - Object is placed in the focal plane (s=f, s'=infinity)
  - Each point of the object forms a parallel bundle
  - A tubelens forms a real image on a camera sensor.

• 2.4 Objective Lenses

• Infinity sign on objective lens

  Optimized for an infinity optical system
• Magnification on objectiive lens

  Total magnification

• 2.5 Numerical aperture

• Numerical aperture of an objective is...

  A measure of its ability to gather light and to resolve detail in a specimen.
• High N.A. Lenses... (2)

  1. Collect more light and form brighter images
  2. Allow to see more detail
• Formula N.A.

  NA = sin mu
• Maximal and best lenses N.A.

  Maximal = 1, Best = 0.95
• Mu increases when... (2)

  1. The aperture of the lens is larger
  2. Distance to the sample is minimized
• Image brightness formula

  NA^2