Förster Resonance Energy Transfer (FRET)
3 important questions on Förster Resonance Energy Transfer (FRET)
Two prerequisites for FRET?
1. Donor must be fluorescent
2. Donor and acceptor must have overlapping spectra (J)
2. Donor and acceptor must have overlapping spectra (J)
What is kappa squared?
The dipole orientation factor --> random = 2/3
What is FRET efficiency?
The efficiently transferred energy of the excited fluorophores, inversely proportional to the sixth power over the distance R between donor and acceptor
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