Light-activated experiments and super-resolution imaging - Super Resolution; beating the diffraction limit - PALM
3 important questions on Light-activated experiments and super-resolution imaging - Super Resolution; beating the diffraction limit - PALM
The principle of Photo Activated Localization Microscopy is...
Activating photoactivatable-GFP by a specific wavelength, after which the temporarily activated fluorophores can be images.
Three disadvantages of PALM are...
1. Bleaching
2. Too long for live-cell imaging
3. Hundreds of cycles needed to collect the final image
2. Too long for live-cell imaging
3. Hundreds of cycles needed to collect the final image
A disadvantage of photoactivatable fluorophores is that...
They bleach faster
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