Fluorescence microscopty - Fluorescence in General
3 important questions on Fluorescence microscopty - Fluorescence in General
What molecules often show fluorescence?
Aromatic molecules, because these are easier excitable
4 light sources for fluorscence are:
1. Halogen/Tungsten lamp; light intensity distributed over a continuous spectrum
2. Gas discharge lamp; Light spectrum with intense peaks
3. LED's; Narrow wavelength band
4. Lasers; High intensity in a single wavelength.
2. Gas discharge lamp; Light spectrum with intense peaks
3. LED's; Narrow wavelength band
4. Lasers; High intensity in a single wavelength.
Why not starte directly into the excitation source?
Because no fliter (excitation filter) blocks 100% effectively.
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