Resolution and contrast generation - Dark field microscopy
3 important questions on Resolution and contrast generation - Dark field microscopy
In DFM, the sample is illuminated with...
A hollow cone of light because a dark-field stop in the back focal plane of the condenser blocks light under small angles
In the absence of a specimen light will continue in straight lines because...
NAObjective < NAcondensor
- This results in that no light from the condesnor will enter the objective lens. Only light that is scattered will form an image through the objective.
- This results in that no light from the condesnor will enter the objective lens. Only light that is scattered will form an image through the objective.
Dark field microscopy principle is that...
Only scattered light will be collected and captured via the objective.
- So, creating contrast by changing the direction of light travel.
- So, creating contrast by changing the direction of light travel.
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