Techniques to determine FRET - Donor Fluorescence Lifetime (FLIM)
3 important questions on Techniques to determine FRET - Donor Fluorescence Lifetime (FLIM)
What is the fluorescence lifetime of a fluorophore?
The average time a fluorophore remains in its excited state before returning to the ground state by emitting a photon.
What is meant with sensitive to the environment?
1. Viscosity, pH, polarity and solvation
2. Size and shape
3. Molecular interactions.
So you can test all these things?
2. Size and shape
3. Molecular interactions.
So you can test all these things?
FRET efficiency of FLIM?
Ratio Tau donor-acceptor / Tau Donor
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