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Summary Advanced Molecular Biology

Course
- Advanced Molecular Biology
- 2021 - 2022
- Radboud Universiteit Nijmegen
- Moleculaire Levenswetenschappen
276 Flashcards & Notes
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A snapshot of the summary - Advanced Molecular Biology

  • 1 LE 1: Molecular Biology Techniques

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  • 1.1 Anlysis techniques

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  • What techniques can be used for specific DNA/RNA analysis?
    Quantitative PCR (qPCR) and Southern & Northern blot
  • Which techniques can be used for analysis of all mRNA/cDNA present?
    Mircoarray and Next Generation Sequencing (NGS)
  • Which technique can be used for analysis of all proteins present?
    Mass-spectrometry
  • Which technique can sort on DNA/RNA content?
    Fluorescence activated cell sorting (FACS)
  • Which techniques can be used to locate proteins?
    Differential/density gradient centrifugation, Immunofluorescence assay (IFA) and Live cell imaging
  • Which techniques can be used to locate DNA and RNA?
    DNA-FISH, RNA-FISH
  • Which techniques can measure the interaction between protein and DNA/RNA?
    Electrophoretic mobility shift assay (EMSA), Chromatin immunoprecipitation (ChIP), RNA immunoprecipitation (RIP)
  • Which teqniques can measure the protein-protein interactions?
    Coimmunoprecipitation (Co-IP), Fluorescent resonance energy transfer (FRET)
  • 1.2 Mutations

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  • Give two characteristics of a gain of function mutation
    1. Substantial increase in protein production of acquisition of a new function
    2. Typically dominant
  • What is targeted mutagenisis?
    Targeted experimental modification of specific gene locus or gene product

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