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A snapshot of the summary - Advanced Molecular Biology
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1 LE 1: Molecular Biology Techniques
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1.1 Anlysis techniques
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What techniques can be used for specific DNA/RNA analysis?Quantitative PCR (qPCR) and Southern & Northern blot
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Which techniques can be used for analysis of all mRNA/cDNA present?Mircoarray and Next Generation Sequencing (NGS)
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Which technique can be used for analysis of all proteins present?Mass-spectrometry
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Which technique can sort on DNA/RNA content?Fluorescence activated cell sorting (FACS)
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Which techniques can be used to locate proteins?Differential/density gradient centrifugation, Immunofluorescence assay (IFA) and Live cell imaging
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Which techniques can be used to locate DNA and RNA?DNA-FISH, RNA-FISH
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Which
techniques can measure theinteraction between protein andDNA /RNA ?Electrophoretic mobility shift assay (EMSA ), Chromatin immunoprecipitation (ChIP), RNA immunoprecipitation (RIP) -
Which teqniques can measure the protein-protein interactions?Coimmunoprecipitation (Co-IP), Fluorescent resonance energy transfer (FRET)
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1.2 Mutations
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Give two characteristics of a gain of function mutation1. Substantial increase in protein production of acquisition of a new function
2. Typically dominant -
What is targeted mutagenisis?Targeted experimental modification of specific gene locus or gene product
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