Read the summary and the most important questions on Advanced Molecular Biology

• 1 LE 1: Molecular Biology Techniques

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• 1.1 Anlysis techniques

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• What techniques can be used for specific DNA/RNA analysis?

  Quantitative PCR (qPCR) and Southern & Northern blot

• Which techniques can be used for analysis of all mRNA/cDNA present?

  Mircoarray and Next Generation Sequencing (NGS)

• Which technique can be used for analysis of all proteins present?

  Mass-spectrometry

• Which technique can sort on DNA/RNA content?

  Fluorescence activated cell sorting (FACS)

• Which techniques can be used to locate proteins?

  Differential/density gradient centrifugation, Immunofluorescence assay (IFA) and Live cell imaging

• Which techniques can be used to locate DNA and RNA?

  DNA-FISH, RNA-FISH

• Which techniques can measure the interaction between protein and DNA/RNA?

  Electrophoretic mobility shift assay (EMSA), Chromatin immunoprecipitation (ChIP), RNA immunoprecipitation (RIP)

• Which teqniques can measure the protein-protein interactions?

  Coimmunoprecipitation (Co-IP), Fluorescent resonance energy transfer (FRET)

• 1.2 Mutations

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• Give two characteristics of a gain of function mutation

  1. Substantial increase in protein production of acquisition of a new function
  2. Typically dominant

• What is targeted mutagenisis?

  Targeted experimental modification of specific gene locus or gene product