Cell cycle II - Cyclin-dependent kinases (Cdk's) and cyclins in the mammalina cell cycle
10 important questions on Cell cycle II - Cyclin-dependent kinases (Cdk's) and cyclins in the mammalina cell cycle
Which type of cylcins contain a destruction box thatr is recognized by the APC ubiquitin ligase?
Name 2 characteristics of cultured G0 cells in the absence of growth factors
2. G0 cells do not progress through the cell cycle and relicate
By which factors is the mammalian cell cylce induced?
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Which two classes of genes are expressed that drive cells back into the cell cylce?
2. Delayed-response genes
Name 3 characteristics of early-response genes
2. Inducton of transcription not blocked by inhibitors of protein synthesis
3. Encode transcription factors that stimulate transcription of delayed-response genes
Name 2 characteristics of delayed-response factors
2. Code for additional transcription factors such as E2Fs, D type cyclins, cyclin E, Cdk2, Cdk4 and Cdk6
On what does passage of the restriction point depend?
What are the 5 steps of regulation of E2f transcription factors?
2. Rb converts E2Fs from trancriptional activators to repressors by recruitng histone deacetylase complexes to DNA-bound E2F
3. Cyclin D-Cdk4/6 will phosphorylate Rb
4. Rb releases E2F
5. E2F can recruit histone acetyltransferases to activate target genes
In what 3 ways can mammalian Cdks be regulated?
2. Phosphorylation and localization of Cdks and cyclins can control Cdk activities
3. Binding of inhibitory proteins
What are the two classes of cyclin-kinase inhibitors of mammals?
2. INK4 (inhibitors of kinase 4) family
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