The Microbial World - Imaging Cells in Three Dimensions
3 important questions on The Microbial World - Imaging Cells in Three Dimensions
To visualize cells in three dimensions, which two different methods can be used?
- Differential Interference Contrast microscopy
- Confocal Scanning Laser microscopy
What is Differential Interference Contrast microscopy?
Differential interference contrast (DIC) microscopy uses a polarizer to create two distinct beams of polarized light. It gives structures such as endospores, vacuoles, and granules a three-dimensional appearance. Structures that are not visible by bright-field microscopy are sometimes visible by DIC.
What is confocal scanning laser microscopy?
Confocal scanning laser microscopy (CSLM) uses a computerized microscope coupled with a laser source to generate a three-dimensional image. A computer can focus the laser on single layers of the specimen. Different layers can then be compiled for a three-dimensional image. The resolution is 0.1 μm for CSLM.
Try our study magic for free
a PDF, study it super fast
- No sign up, email or credit card needed!
- AI makes unlimited flashcards
- Get unlimited quizzes and tests
- Ask AI anything
Create a notebook
- No sign up, email or credit card needed!
- Have and keep perfect overview
- Make flashcards, notes and mind maps
- Review, test and score!
The question on the page originate from the summary of the following study material:
- A unique study and practice tool
- Never study anything twice again
- Get the grades you hope for
- 100% sure, 100% understanding
Remember faster, study better. Scientifically proven.
