Chromatin organization and fuction biochemistry and molecular biology
17 important questions on Chromatin organization and fuction biochemistry and molecular biology
How is chromatin regulated?
2. Replacement of histone isoform.
3. Post translational modifications.
What post translational modifications of proteins are there?
2. Addition of other proteins.
3. Chemical modification of amino acid side chains. --> chirality
4. Structural changes. --> disulphide bridges
How does phosphorylation affect proteins?
3 possibilities for H-bonds
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Where on histones happen the post-translational changes?
What changes in lysine acetylation?
How can changes in the protein affect the function?
Any modificaton on lysine and arginine will change the interaction between histones and DNA.
2. Binding site for other proteins.
How important are the tails in stabilyzing the chromatin?
How can PTMs change the interactions with other proteins?
How does the integration of multiple PTMs work?
prot A binds mod 1, prot B binds mod 2, prot C binds A and B.
Tails are connected.
What are the functional consequences of H3K4 methylation?
2. It is recognized by ING5 opening the DNA for replication.
What is the function of chromatin?
- regulation of expression
- cellular memory (type of cell)
- learning and memory
- related diseases
- ageing
- influence of environment
What does chromatin exist of?
What does one nucleosome consist of?
What is the role of histone 1?
What is ATP-dependent chromatin remodeling complex?
How do repressors and activators effect gene expression?
Activators can decondense chromatin, repressors can condense chromatin.
Difference histone and DNA methylation?
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