Markus Affolter - Dorsal closure
3 important questions on Markus Affolter - Dorsal closure
Goal of Markus' paper
Acute force elimination via selective MyosinII depletion in one or the other tissue shows that the amnioserosa tissue autonomously drives dorsal closure while the actin/MyosinII cable cannot.
Sqh-GFP KO of epidermal actin/myosin cable (ES)
Dorsal closure!
How did they selectively remove MyosinII activity in each tissue, prior to dorsal closure?
By targeting GFP-tagged MyosinII light chain Sqh (Sqh-GFP) to protein degradation using UAS/Gal4- mediated deGradFP expression.
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