Gene regulation in bacteria - Regulation of the trp operon
14 important questions on Gene regulation in bacteria - Regulation of the trp operon
How is the trp operon regulated?
- By a mechanism called attenuation.
Which kind of sequences does the trp operon consist of?
- promoter;
- operator;
- genes: trpE, trpD, trpC, trpB, and trpA.
What happens to the repressor when the tryptophan levels within the cell are very low?
- RNA polymerase transcribes the trp operon;
- the cell expresses the genes in the trp operon.
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What happens to the repressor when the tryptophan levels within the cell are high?
- a conformational change in the repressor occurs;
- repressor binds to operator site;
- the ability of RNA polymerase to transcribe the trp operon is inhibited.
What is the concept of attenuation?
Where is the transcription stopped during attenuation?
What are the two key features of attenuation?
- The mRNA made from the trpL gene has four regions that are complementary to each other, so that the formation of stem loops is possible.
To what does a stem-loop in regions 3 and 4 lead?
In which kind of operons does attenuation often occur?
What kind of operons are lac operon and trp operon with regard to how they are regulated?
What kind of genes are often regulated in an inducible manner?
What kind of genes are often regulated in a repressible manner?
Why is gene regulation in genes that code for proteins involved in the breakdown of substances useful?
Why is gene regulation in genes that encode for anabolic enzymes useful?
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