Research on Nephropathology
11 important questions on Research on Nephropathology
What to do (therapy) with a leaky glomerulus/nepron?
Why do we need different antibodies of different species to stain different colors?
Name two new insights in nephrotic syndrome?
- Glomerular filtration barrier; 'the electrokinetic model'
- Development of FSGS lesions: 'the role of parietal epithelial cells' immunohistological stainings
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Glomerular filtration barrier
cause: leaky filter
What makes the glomerular filtration barrier charge and size selective? Why does the filter never clog?
What determines the glomerular permeability?
What morphological change of the podocyte foot processies cause proteinuria? And what else can it cause?
proteinuria: loss of filtration lead to an increased permeability (loss of electric potential)
What is the electrokinectic model?
- no clogging of the filter
- it allows negative charged growth factors to travel from podocyte to endothelial cells i.e. VEGF
- effacement of the foot processes wil disturb the flow through the filter and thus the electrokinetic effects
Focal segmental glomerulosclerosis (FSGS)
How is the role of PECs in focal segmental glomerulosclerosis studied?
- complex anatomy of the kidney
- focal and segmental aspects of the disease
- co-staining of multiple markers
What are three possible problems with immunological staining that one should keep in mind?
- many markers are less specific as mentioned in literature
- specificity may change due to cell change in for instance pathological processes
- e.g. High resemblance between immunoglobulins form mouse and rat. This may and lead to cross-reactivity when using both rat and mouse antibodies.
- e.g. Cryo-section or paraffin section, fixative, antigen retrieval
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