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Tissue preparation & techniques

Q: Is it normal to see a capillary loop in the lumen of a proximal tubule?

No it's not normal and not seen often, it's a artefact during biopsy

17 important questions on Tissue preparation & techniques

In which position should you place the condenser for a stained and unstained slide?

unstained slight condenser in lowest position ( to get more contrast)
stained in highest position

Where can we find the artery arcuate and which kind of tissue does it consist of?

Is located between the cortex and medulla. Consists of continuous endothelium.

Is there shrinkage of tissue during drying?

Yes there is shrinkage, every cell/tissue (with water in it) has shrinkage during drying.

A human kidney slide with 20 glomeruli will show at least 15 planes with a tubular pole, true or false?

False, much less, only few percent

Is it normal to see a capillary loop in the lumen of a proximal tubule?

No it's not normal and not seen often, it's a artefact during biopsy

Can a slide of solitary cells (puncture or smear) provide the same information as an tissue (histology) slide?

False, in solitary cells you see several characteristics, (e.g. You see severe displastic Cells). In a tissue slide you can't see where these cells come from (?). So in a smear you can see very much detail.

What is the function of fixation of tissue

To stop autolysis and prevent cell from getting different morphology.

For a good image you need a high antigenicity, how can this be achieved?

You need to use a mild fixation.

Antigenicity = describes the ability of a foreign material (antigen) to bind to, or interact with, the products of the final cell-mediated response

Which slides have better resolution? Thinner or thicker slides?

Thinner slides have a higher resolution.
But the activity (of proteins?) is much higher in a thicker slide (and resolution lesser)

Eosin and ... Hematoxylin (HE stain) are most common used staining, what do you stain?

.... See ppt slides cytoplasm?

Connection of GBM and bowman's capsule means?

called a senegy, The first step of sclerosis (?) check effe

Why do we see more with microscopic microscope then with light microscope?

Because in the formula of the resolution, so because of the wavelength and the NA (numeric aperture)

Resolution = K x wavelength / NA (effe checken)

so green light has a better resolution than red light, because green has a shorter wave length.

Shorter wave length = smaller R = better resolution

With a cryo fixation the antigenicity will improve, true or false?

True. Proteins very active, so antigenicity very high

What is the real disadvantage of cryo fixation?

Ice Cristals, cryodamage. -> when you cryo fixation technique is not fast enough.

good solution but also has disadvantage (expensive etc) is the below:

High pressure freezing (at higher pressure water will not freeze at 0 C but at -40 c (zoiets??) so you have much more time. Nice to get a high immune histochemistry and thus high antigenicity.

What do we detect with CLSM (confocal laser scanning microscopy)?

The pine whole only shows what is sharp.

when the pinhole is increased in CLSM it turns in a normal standard fluorescence microscopy, because not only what is sharp is shown anymore

What can we measure with EDX = X-ray scanning microscopy (orzo?)

You can measure different elements of periodic table, and can give you some info about the e.g. Kidney stone build up.

So you can only measure elements but not the structural formula of a substance.

Which tissue samples sorts are there?

Frozen
LM
EM
Specific (EM Pact-HPF)
Molecular Applications

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Human Pathology

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Tissue preparation & techniques

17 important questions on Tissue preparation & techniques

In which position should you place the condenser for a stained and unstained slide?

Where can we find the artery arcuate and which kind of tissue does it consist of?

Is there shrinkage of tissue during drying?

A human kidney slide with 20 glomeruli will show at least 15 planes with a tubular pole, true or false?

Is it normal to see a capillary loop in the lumen of a proximal tubule?

Can a slide of solitary cells (puncture or smear) provide the same information as an tissue (histology) slide?

What is the function of fixation of tissue

For a good image you need a high antigenicity, how can this be achieved?

Which slides have better resolution? Thinner or thicker slides?

Eosin and ... Hematoxylin (HE stain) are most common used staining, what do you stain?

Connection of GBM and bowman's capsule means?

Why do we see more with microscopic microscope then with light microscope?

With a cryo fixation the antigenicity will improve, true or false?

What is the real disadvantage of cryo fixation?

What do we detect with CLSM (confocal laser scanning microscopy)?

What can we measure with EDX = X-ray scanning microscopy (orzo?)

Which tissue samples sorts are there?

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