Laboratory techniques - Isolation of an antigen
3 important questions on Laboratory techniques - Isolation of an antigen
What are the steps in immunoprecipitation?
1. Start with the mixture of antigen of interest
2. Add excess immobilised antibody specific for antigen of interest
3. Collect immobilised antibody by centrifugation
4. Wash with fresh solution to remove unbound antigens
5. Denature antibody to elute antigen
2. Add excess immobilised antibody specific for antigen of interest
3. Collect immobilised antibody by centrifugation
4. Wash with fresh solution to remove unbound antigens
5. Denature antibody to elute antigen
What is affinity chromatography used for?
It is used to isolate soluble antigens or antibodies specific for an immobilised antigen
What are the steps in affinity chromatography?
1. Anti-X antibody bound to insoluble beads
2. Add solution with mixture of antigens
3. Wash away unbound antigens
4. Elute antigen X
2. Add solution with mixture of antigens
3. Wash away unbound antigens
4. Elute antigen X
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