Transitions between cellular states - Cell state transition in development
5 important questions on Transitions between cellular states - Cell state transition in development
Out of what is the inner cell mass of a blastocyst made?
- Epiblast contributes to the embryo
- Blastocyst contributes to muscle, blood, nerve, cardiac, liver, intestinal cells
What are the differentiation potential of Stem cells?
- Totipotency: able to differentiate in all cell types, both embryonic (stem cells) and extra-embryonic (placenta)
- Pluripotency: able to differentiate in all cell lineage of the body (ES and PGS: geslachtcellen)
- Multipotency: multiple cells types of one lineage (endoderm, mesoderm, ectoderm)
- Unipotency: cells from one cell type only (neurones, gut cells, ...)
What are the therapeutic promises of stem cell research?
- immune system rejects "stranger" cells
- Ethical argument to not use human embryo's for experiment/research
- Higher grades + faster learning
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What is the solution of embryonic stem cell use?
- the fibroblast cell comes out of the patient and is reprogrammed into an embryonic stem cell, so no immune reaction on iPS
- ethic arguments are gone, because no embryo is used
What are the mechanism of iPS?
- Nucleus out of oocyte (ovary) and the nucleus of a somatic cell (fibroblast) of the patient implanted in the oocyte
- creates a blastocyst which has ES cells
- somatic cells can be reprogrammed into embryonic state
- start with patient fibroblast
- multiple TF used to transform the somatic cell into an induced pluripotent stem cell (iPS cell)
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