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Summary Powerpoints

Course
- Bioengineering and -omics
- Sura
- 2020 - 2021
- VHL University of Applied Sciences (VHL University of Applied Sciences locatie Leeuwarden, Leeuwarden)
- Biologie en Medisch Laboratoriumonderzoek
126 Flashcards & Notes
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A snapshot of the summary - Powerpoints

  • 1 Refreshment of molecular biology & Instruction practicum

  • 1.1 Restriction enzymes

    This is a preview. There are 12 more flashcards available for chapter 1.1

  • What is a restriction enzyme?
    A protein that recognizes palindromatic DNA sequences
  • What are the names of restriction enzymes based on?
    The bacteria from which they are isolated.
  • What is a characteristic of sticky ends ligation?
    It creates an overhang. The overhangs, called "sticky ends", are what allow the vector and insert to bind to each other.
  • What is a downside of blunt ends ligation?
    It is less stringent than sticky end ligation.
  • What do you call enzymes that recognize the same sequence, but are from different organisms?
    Isoschizomers.
  • What are type I enzymes?
    Complex, multisubunit, combination restriction-and-modification enzymes that cut DNA at random far from their recognition sequences.
  • What are type IIS enzymes?
    Those that cleave outside of their recognition sequence to one side.
  • What are type IV enzymes?
    Recognize modified, typically methylated DNA.
  • 1.2 Plasmids

    This is a preview. There are 12 more flashcards available for chapter 1.2

  • What is a plasmid?
    A small circular double stranded DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently.
  • What are cloning plasmids used for?
    To facilitate the cloning of DNA fragments.

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