Strategies of genetic engineering - Homologous recombination
3 important questions on Strategies of genetic engineering - Homologous recombination
What is homologous recombination (HR)?
An essential pathway to restart stalled replication forks, repair spontaneous DNA double-strand breaks, and generate genetic diversity.
The ends of DNA are homologous (same) to the chromosomal DNA or plasmid DNA.
The ends of DNA are homologous (same) to the chromosomal DNA or plasmid DNA.
What is non-homologous end-joining (NHEJ)?
A DSB repair pathway that ligates or joins two broken ends together, resulting in the introduction of small insertions or deletions (indels) at the site of the DSB.
What is homology directed repair (HDR)?
A DNA template-dependent pathway for DSB repair, using a homology-containing donor template along with a site-specific nuclease, enabling the insertion of single or multiple transgenes (gene addition) in addition to single-nucleotide substitutions in which an amino acid substitution of a protein occurs (gene modification), or a mutation is completely repaired in the resultant organism genome (gene correction).
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