Zebrafish models assessing developmental and reproductive toxicity
8 important questions on Zebrafish models assessing developmental and reproductive toxicity
What are the advantages of the zebrafish use in toxicology research?
- high througput
- short test period
- low cost
- small amount of compound required
- easy manipulation
- direct compound delivery
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What are the 4 toxic endpoints of zebra fish death?
- failure to develop somites (segments)
- lack of heartbeat
- non-attachment of the tail from the yolk.
What are current alternatives for exposing rats and rabbits when they are pregnant to drugs?
- in vitro rodent whole embryo culture test
- in vivo frog embryo teratogenesis assay (FETAX)
but these in vitro and in vivo test are not sensitive and are of a limited value in predicting the effect of drugs and chemicals on human embryonic and fetal development.
Again, what are the reasons why the zebra fish model is widely accepted?
2) the developmental processes in the zebrafish are highly conserved
3) zebra fish embryos can be cultured until advanced embryogenesis in contrast to rodents.
4) genome is well characterized.
Assessing reproductive toxicity in zebra fishes is focused on?
2) reproduction ability (the egg laying amount)
3) the gonad histology
4) the vitellogenin espression assay
5) hypothalamus-pituitary-gonad axis gene expression analysis. (some compounds can disrupt this system)
How is behavioral toxicity meassured in the zebra fish?
- an assay using the visual stimulus because zebra fishes are social animals that communicate via colors.
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